Increasing polyhydroxyalkanoate (PHA) yields from Cupriavidus necator by using filtered digestate liquors

Pearl Passanha*, Sandra R. Esteves, Gopal Kedia, Richard M. Dinsdale, Alan J. Guwy

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)


The production of polyhydroxyalkanoates (PHAs) using digestate liquor as culture media is a novel application to extend the existing uses of digestates. In this study, two micro-filtered digestates (0.22. μm) were evaluated as a source of complex culture media for the production of PHA by Cupriavidus necator as compared to a conventional media. Culture media using a mixture of micro-filtered liquors from food waste and from wheat feed digesters showed a maximum PHA accumulation of 12.29. g/l PHA, with 90% cell dry weight and a yield of 0.48. g PHA/g VFA consumed, the highest reported to date for C. necator studies. From the analysis of the starting and residual media, it was concluded that ammonia, potassium, magnesium, sulfate and phosphate provided in the digestate liquors were vital for the initial growth of C. necator whereas copper, iron and nickel may have played a significant role in PHA accumulation.

Original languageEnglish
Pages (from-to)345-352
Number of pages8
JournalBioresource Technology
Early online date14 Aug 2013
Publication statusPublished - Nov 2013


  • Digestate liquors
  • Nutrients
  • Polyhydroxyalkanoate production
  • Trace metals
  • Volatile fatty acids


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