Targeting of Receptor Activator of Nuclear Kappa B (RANK) in PC-3 Cells Increases Cell Proliferation and Matrix Adhesion In Vitro

Sioned Owen*, Andrew J. Sanders, Malcolm D. Mason, Wen G. Jiang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Background: In Western societies, prostate cancer is the most frequently diagnosed cancer amongst men. Efforts to improve diagnosis and treatment remain a major focus and have been proven beneficial in the approach to localised disease. However, currently, metastatic disease management still remains palliative. Receptor activator of nuclear kappa B (RANK) has been extensively studied in bone biology and immunology, whilst several links have been made between RANK-positive breast cancer cells and disease progression. Its role in prostate cancer biology remains poorly understood, therefore the aim of this study was to explore the functional role of endogenously produced RANK in metastatic PC-3 prostate cancer cells in isolation and in response to hepatocyte growth factor (HGF). Materials and Methods: RANK expression was targeted using hammerhead ribozyme technology in PC-3 prostate cancer cells, and verified by polymerase chain reaction and western blot. A variety of in vitro functional assays were conducted, including cell proliferation and matrix adhesion in the presence of HGF. Results: Suppression of RANK expression was successfully targeted with anti-RANK hammerhead ribozyme transgenes, as verified by PCR and western blot. Reduced RANK expression resulted in significantly increased PC-3 cell proliferation (p

Original languageEnglish
Pages (from-to)1127-1134
Number of pages8
JournalAnticancer research
Issue number3
Publication statusPublished - Mar 2016
Externally publishedYes
EventChina-United Kingdom Cancer (CUKC) Conference 2015 - National Museum, Cardiff, United Kingdom
Duration: 17 Jul 201518 Jul 2015


  • Prostate cancer
  • PC-3
  • RANK
  • HGF
  • C-MET


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